Immunohistochemical staining:Article Title: Progenitor exhausted PD-1 + T cells are cellular targets of immune checkpoint inhibition in atherosclerosis.
Article Snippet: .. For immunohistochemical staining, aortic root cross-sections were stained with the following: anti-CD3 (Armenian hamster anti-mouse, BioLegend) as primary antibody, biotinylated goat anti-hamster (Vector Laboratories) as secondary antibody and Armenian hamster IgG2a (BioLegend) as isotype control; anti-PD-L1 (rat anti-mouse, BioLegend) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2b (Abcam) as isotype control; anti-CD19 antibody (rabbit anti-mouse, Abcam), biotinylated anti-rabbit IgG as secondary antibody and rabbit IgG as isotype control; anti-Ly6G antibody (clone: 1A8, rat anti-mouse IgG, BD Biosciences) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control; and anti-CD68 (clone: FA-11, rat anti-mouse IgG, Bio-Rad) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control. .. For FFPE hearts, antigen retrieval was performed using sodium citrate buffer (pH 6) before continuing with primary antibody staining (anti-CD3, clone:SP7, Abcam) followed by ImmPRESS HRP goat anti-rabbit (Vector Laboratories).
Article Title: Progenitor exhausted PD-1 + T cells are cellular targets of immune checkpoint inhibition in atherosclerosis
Article Snippet: .. For immunohistochemical staining, aortic root cross-sections were stained with the following: anti-CD3 (Armenian hamster anti-mouse, BioLegend) as primary antibody, biotinylated goat anti-hamster (Vector Laboratories) as secondary antibody and Armenian hamster IgG2a (BioLegend) as isotype control; anti-PD-L1 (rat anti-mouse, BioLegend) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2b (Abcam) as isotype control; anti-CD19 antibody (rabbit anti-mouse, Abcam), biotinylated anti-rabbit IgG as secondary antibody and rabbit IgG as isotype control; anti-Ly6G antibody (clone: 1A8, rat anti-mouse IgG, BD Biosciences) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control; and anti-CD68 (clone: FA-11, rat anti-mouse IgG, Bio-Rad) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control. .. For FFPE hearts, antigen retrieval was performed using sodium citrate buffer (pH 6) before continuing with primary antibody staining (anti-CD3, clone:SP7, Abcam) followed by ImmPRESS HRP goat anti-rabbit (Vector Laboratories).
Staining:Article Title: Progenitor exhausted PD-1 + T cells are cellular targets of immune checkpoint inhibition in atherosclerosis.
Article Snippet: .. For immunohistochemical staining, aortic root cross-sections were stained with the following: anti-CD3 (Armenian hamster anti-mouse, BioLegend) as primary antibody, biotinylated goat anti-hamster (Vector Laboratories) as secondary antibody and Armenian hamster IgG2a (BioLegend) as isotype control; anti-PD-L1 (rat anti-mouse, BioLegend) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2b (Abcam) as isotype control; anti-CD19 antibody (rabbit anti-mouse, Abcam), biotinylated anti-rabbit IgG as secondary antibody and rabbit IgG as isotype control; anti-Ly6G antibody (clone: 1A8, rat anti-mouse IgG, BD Biosciences) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control; and anti-CD68 (clone: FA-11, rat anti-mouse IgG, Bio-Rad) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control. .. For FFPE hearts, antigen retrieval was performed using sodium citrate buffer (pH 6) before continuing with primary antibody staining (anti-CD3, clone:SP7, Abcam) followed by ImmPRESS HRP goat anti-rabbit (Vector Laboratories).
Article Title: Progenitor exhausted PD-1 + T cells are cellular targets of immune checkpoint inhibition in atherosclerosis
Article Snippet: .. For immunohistochemical staining, aortic root cross-sections were stained with the following: anti-CD3 (Armenian hamster anti-mouse, BioLegend) as primary antibody, biotinylated goat anti-hamster (Vector Laboratories) as secondary antibody and Armenian hamster IgG2a (BioLegend) as isotype control; anti-PD-L1 (rat anti-mouse, BioLegend) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2b (Abcam) as isotype control; anti-CD19 antibody (rabbit anti-mouse, Abcam), biotinylated anti-rabbit IgG as secondary antibody and rabbit IgG as isotype control; anti-Ly6G antibody (clone: 1A8, rat anti-mouse IgG, BD Biosciences) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control; and anti-CD68 (clone: FA-11, rat anti-mouse IgG, Bio-Rad) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control. .. For FFPE hearts, antigen retrieval was performed using sodium citrate buffer (pH 6) before continuing with primary antibody staining (anti-CD3, clone:SP7, Abcam) followed by ImmPRESS HRP goat anti-rabbit (Vector Laboratories).
Control:Article Title: Progenitor exhausted PD-1 + T cells are cellular targets of immune checkpoint inhibition in atherosclerosis.
Article Snippet: .. For immunohistochemical staining, aortic root cross-sections were stained with the following: anti-CD3 (Armenian hamster anti-mouse, BioLegend) as primary antibody, biotinylated goat anti-hamster (Vector Laboratories) as secondary antibody and Armenian hamster IgG2a (BioLegend) as isotype control; anti-PD-L1 (rat anti-mouse, BioLegend) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2b (Abcam) as isotype control; anti-CD19 antibody (rabbit anti-mouse, Abcam), biotinylated anti-rabbit IgG as secondary antibody and rabbit IgG as isotype control; anti-Ly6G antibody (clone: 1A8, rat anti-mouse IgG, BD Biosciences) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control; and anti-CD68 (clone: FA-11, rat anti-mouse IgG, Bio-Rad) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control. .. For FFPE hearts, antigen retrieval was performed using sodium citrate buffer (pH 6) before continuing with primary antibody staining (anti-CD3, clone:SP7, Abcam) followed by ImmPRESS HRP goat anti-rabbit (Vector Laboratories).
Article Title: Progenitor exhausted PD-1 + T cells are cellular targets of immune checkpoint inhibition in atherosclerosis
Article Snippet: .. For immunohistochemical staining, aortic root cross-sections were stained with the following: anti-CD3 (Armenian hamster anti-mouse, BioLegend) as primary antibody, biotinylated goat anti-hamster (Vector Laboratories) as secondary antibody and Armenian hamster IgG2a (BioLegend) as isotype control; anti-PD-L1 (rat anti-mouse, BioLegend) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2b (Abcam) as isotype control; anti-CD19 antibody (rabbit anti-mouse, Abcam), biotinylated anti-rabbit IgG as secondary antibody and rabbit IgG as isotype control; anti-Ly6G antibody (clone: 1A8, rat anti-mouse IgG, BD Biosciences) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control; and anti-CD68 (clone: FA-11, rat anti-mouse IgG, Bio-Rad) as primary antibody, biotinylated rabbit anti-rat (Vector Laboratories) as secondary antibody and rat IgG2a as isotype control. .. For FFPE hearts, antigen retrieval was performed using sodium citrate buffer (pH 6) before continuing with primary antibody staining (anti-CD3, clone:SP7, Abcam) followed by ImmPRESS HRP goat anti-rabbit (Vector Laboratories).
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